pFUSE-mIgG1e2-Fc
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pFUSE-mIgG1e2-Fc1
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Show product |
20 µg |
pfc1-mg1e2
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pFUSE-mIgG1e2-Fc2
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Show product |
20 µg |
pfc2-mg1e2
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pFUSE-mIgG1e2-Fc1 is a cloning plasmid for the generation of Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG1 heavy chain with the T252M mutation and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-mIgG1e2-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Nagaoka M. & Akaike T., 2003 Single amino acid substitution in the mouse IgG1 Fc region induces drastic enhancement of the affinity to protein A. Protein Eng. 16(4):243:245.
Specifications
Isotype : IgG1 mouse
Mutation: T252M
Characteristics:
- Increased protein A affinity
- Reduced CDC, no ADCC
Both plasmids are selectable with Zeocin™.
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- 20 µg of lyophilized DNA
- 1 ml of Zeocin® (100 mg/ml)
Product is shipped at room temperature