pFUSE-CHIg-hG1e5

pFUSE-CHIg general plasmid map

Human IgG1 heavy chain constant region with increased ADCC

pFUSE-CHIg-hG1e5 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with S239D / A330L / I332E mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e5 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pFUSE-CHIg-hG1e5

• Encodes the human IgG1e5 CH constant region (S239D / A330L / I332E mutations)
• Long-lasting expression due to the composite hEF1-HTLV promoter
• Selectable with Zeocin™ in both bacterial and mammalian cells
• Available with or without the IL-2 signal sequence

References:

1. Lazar GA. et al., 2006. Engineered antibody Fc variants with enhanced effector function. PNAS 103(11): 4005–4010.
2. Ryan MC. et al., 2007. Antibody targeting of B-cell maturation antigen on malignant plasma cells. Mol. Cancer Ther. 6: 3009 - 3018.
3. Oganesyan V. et al., 2007. Structural characterization of a mutated, ADCC-enhanced human Fc fragment. Mol. Immunol. 45(7): 1872-82.

Specifications

• pFUSE-CHIg-hG1e5 features the constant region of the human IgG1 heavy chain with S239D / A330L / I332E mutations

• pFUSEss-CHIg-hG1e5 features the constant region of the human IgG1 heavy chain with S239D / A330L / I332E mutations and the IL2 signal sequence

Characteristics of S239D / A330L / I332E mutations:
- Increased binding to FcγRIIIa
- Increased ADCC

Both plasmids are selectable with Zeocin™.

pFUSE Contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin® (100 mg/ml)

Product is shipped at room temperature