pDRIVE-SP-B
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-hSPB pDRIVE bearing human SP-B promoter - LacZ |
Show product |
20 µg |
pdrive-hspb
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pDRIVE5SEAP-hSPB pDRIVE bearing human SP-B promoter - SEAP |
Show product |
20 µg |
pdrive5s-hspb
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pDRIVE5Lucia-hSPB pDRIVE bearing human SP-B promoter - Lucia |
Show product |
20 µg |
pdrive5lc-hspb
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SP-B promoter (Lung) in pDRIVE expression plasmid
Surfactant protein B (SP-B) is a developmentally and hormonally regulated lung protein that is required for normal surfactant function. SP-B is selectively expressed in bronchiolar and alveolar epithelial cells of the lung.
The human SP-B promoter fragment directs tissue- and lung cell-specific transgene expression [1].
It contains two cis-acting elements for two transcription factors, thyroid transcription factor (TTF)-1 and hepatocyte nuclear factor (HNF)-3 that are important for the specificity and the activation of gene expression in the lung [2].
Promoter activity is strongly inhibited in a dosedependent fashion by phorbol ester, such as 12-O-tetradecanoylphorbol-13-acetate (TPA).
References:
1. Strayer M. et al. 2002. Human surfactant protein B promoter in transgenic mice: temporal, spatial, and stimulus-responsive regulation. Am J Physiol Lung Cell Mol Physiol 282(3):L394-404.
2. Kumar AS. et al., 1997. Phorbol ester down-regulation of lung surfactant protein B gene expression by cytoplasmic trapping of thyroid transcription factor-1 and hepatocyte nuclear factor 3. J Biol Chem. 72(33):20764-73.
Specifications
pDRIVE bearing human SP-B promoter - LacZ, SEAP or Lucia
Gene Promoter: Surfactant Protein B
Specificity: Lung
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
