pDRIVE-NSE / RU5′

Specifications

pDRIVE bearing rat NSE / RU5' promoter - LacZ, SEAP or Lucia
Gene Promoter: NSE : Neuron-Specific Enolase
Specificity: Mature neurons
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™

Contents

- Provided as 20 µg lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar).

Description

Neuron-specific enolase, an isoenzyme form of enolase, occurs in mature neurons and paraneurons. Transgenic mouse studies have shown that a 1.8 kb fragment of the rat NSE promoter is able to express a heterologous gene exclusively in postmitotic neurons and neuro-endocrine cells in parallel with endogenous NSE [1]. The NSE promoter contains a TATA-like sequence, no CAAT box and sequences for the AP-1 binding motif, AP-2 binding element, SP-1 binding sequence and cAMP response element [2]. The NSE promoter was used to create transgenic mice exhibiting the neuropathological phenotypes of Alzheimer's disease [3].

1. Forss-Petter S. et al. 1990. Transgenic mice expressing beta-galactosidase in mature neurons under neuron-specific enolase promoter control. Neuron 5(2):187-97.
2. Sakimura K. et al. 1995. Upstream and intron regulatory regions for expression of the rat neuron-specific enolase gene. Brain Res Mol B 28(1):19-28.
3. Hwang DY. et al., 2004. Aberrant expressions of pathogenic phenotype in Alzheimer's diseased transgenic mice carrying NSE-controlled APPsw. Exp Neurol. 186(1):20-32.

Details

pDRIVE map

pDRIVE5-SEAP map

ORDERING

pDRIVE-rNSE-RU5'

Description pDRIVE bearing rat NSE / RU5' promoter - LacZ
Cat. Code pdrive-rnseru5
Unit Size 20 µg
Price For price or distributor address,
please select your country

pDRIVE5SEAP-rNSE-RU5'

Description pDRIVE bearing rat NSE / RU5' promoter - SEAP
Cat. Code pdrive5s-rnseru5
Unit Size 20 µg
Price For price or distributor address,
please select your country

pDRIVE5Lucia-rNSE-RU5'

Description pDRIVE bearing rat NSE / RU5' promoter - Lucia
Cat. Code pdrive5lc-rnseru5
Unit Size 20 µg
Price For price or distributor address,
please select your country
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