CpG-free plasmid for siRNA production in vivo

pCpGfree-siRNA is a plasmid that combines the backbone of the pCpGfree plasmids with the siRNA expression cassette of the psiRNA plasmids. It is designed for long lasting expression of shRNAs in vivo as the plasmid does not induce inflammatory responses and gene silencing by methylation in vertebrate hosts.


CpG-free plasmid backbone

Two cloning options: Acc 65I / Hind III or Bbs I / Bbs I

Allows white and blue selection

Selectable in E. coli with Zeocin™

Provided with the psiRNA™ tools:
• disk of lyophilized E. coli GT115
• Sequencing primers (OL559-OLSI3')
E. coli Fast-Media® Zeo XGal

This product is covered by a Limited Use License (See Terms and Conditions).


pCpGfree-siRNA plasmid is provided in a kit that includes the following components:

- 50 μg pCpGfree-siRNA plasmid
- 20 μg control pCpGfree-siRNA plasmid expressing a scramble sequence
- 1 disk of lyophilized E. coli GT115 (genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZM15 ΔlacX74 recA1 endA1 Δdcm uidA::pir-116 ΔsbcC-sbcD)
- 10 μg of OL559 primer (forward) and 10 μg of OLSI3' primer (reverse)
- 4 pouches of E. coli Fast-Media® Zeo X-Gal


CpG-free Plasmid Backbone
All the elements required for replication and selection of the plasmid in E. coli and gene expression in mammalian cells are completely devoid of CpG dinucleotides.

Enhanced RNA Polymerase III Promoter
pCpGfree-siRNA plasmid features the human 7SK promoter, in which all CpG dinucleotides have been removed. The activity of this RNA Pol III promoter is augmented by the addition of the enhancer of the CMV immediate-early promoter, a Pol II enhancer. The enhancer has been modified to remove all CpGs.

White/blue selection: pCpGfree-siRNA plasmids exploit the white/blue selection system to facilitate the screening of recombinant clones. They feature an EM7-LacZ α-peptide cassette between the cloning sites to allow the easy discrimination between blue parental and white recombinant clones.

Cloning options: pCpGfree-siRNA offers two cloning strategies for the cloning of an shRNA insert, either Acc 65I / Hind III or Bbs I / BbsI. Bbs I, an unusual restriction enzyme that generates asymmetric cohesive overhangs that are not compatible with each other to eliminate the risk of self-ligation of the vector.


pCpGfree-siRNA backbone



Description CpG-free plasmid designed for the production of siRNAs in vivo
Cat. Codekcpgf-sirna
Unit Size1 kit
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".