ODN 2216 Biotin

Biotin labeled CpG oligonucleotide - Human TLR9 ligand

CpG  ODNs are synthetic oligonucleotides that contain unmethylated CpG  dinucleotides in particular sequence contexts (CpG motifs). These CpG  motifs are present at a 20-fold greater frequency in bacterial DNA  compared to mammalian DNA. They have been shown to induce a coordinated  set of immune responses based on the activation of immune cells  primarily involved in the recognition of these molecules.

ODN 2216 Biotin is a A-class CpG ODN with a preference for human TLR9. ODN 2216 Biotin can be used to evaluate CpG ODN cellular uptake and localization using a biotin detection system and light microscopy.

Contents update notification

February, 3rd 2017
ODN 2216 Biotin is provided as a 100 µg (2 x 50 µg) unit size (previously sold as a 50-µg unit size). This product is now sold at a more competitive price.


Specificity: Human TLR9 agonist

Working concentration: 1-5 μM

ODN 2216 sequence
5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer)
Note: Bases shown in capital letters are phosphodiester, and those in lower case are phosphorothioate (nuclease resistant). Palindrome is underlined.


• 2 x 50 μg (7 nmol) lyophilized ODN 2216 labeled with Biotin at the 3’ terminus
• 1 ml sterile endotoxin-free water

ODN 2216 Biotin is shipped at room temperature and should be stored at -20°C. Lyophilized ODN 2216 Biotin is stable for at least 1 year. Upon resuspension, prepare aliquots of ODN 2216 Biotin and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.


ODN 2216 Biotin

Description Class A - Biotinylated CpG ODN 2216
Cat. Codetlrl-2216b
Unit Size2 x 50 µg
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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