Specifications

Specificity: Th1 response

Working concentration: 5 - 30 μg/mouse

Endotoxin level: <1 EU/mg

Solubility: 5 mg/ml in physiological water

Documents

• TDS N-Glycolyl-MDP VacciGrade : 5 mg (vac-gmdp)
• MSDS N-Glycolyl-MDP VacciGrade : 5 mg (vac-gmdp)

Contents

• 5 mg N-glycolyl-MDP VacciGrade™
• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

N-glycolyl-MDP is provided as a sterile white lyophilized powder and shipped at room temperature. Store at -20°C. Lyophilized product is stable for 1 year at -20°C. Upon resuspension, N-glycolyl-MDP should be aliquoted and stored at -20°C. Resuspended product is stable for 6 months at -20°C when properly stored. Avoid repeated freeze-thaw cycles.

Description

MDP (muramyl dipeptide), is the minimal bioactive peptidoglycan motif common to all bacteria and the essential structure required for adjuvant activity in vaccines.

MDP has been shown to be recognized by NOD2, but not TLR2, nor TLR2/1, or TLR2/6 associations [1].

The cell wall of mycobacteria is known to be extremely immunogenic. This potent activity is attributed to their MDP which is N-glycolylated in contrast to the MDP of most bacteria which is N-acetylated. N-glycolyl-MDP has been reported to display a stronger NOD2-mediated activity than N-acetyl-MDP and thus to be a more potent vaccine adjuvant than N-acetyl-MDP [2]. Furthermore MDP leads to the activation of the NLRP3 inflammasome [3].

1. Girardin S. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
2. Coulombe F. et al., 2009. Increased NOD2-mediated recognition of N-glycolyl muramyl dipeptide. J Exp Med. 206(8):1709-16.
3. Martinon F. et al., 2004. Identification of bacterial muramyl dipeptide as activator of the NALP3/cryopyrin inflammasome. Curr Biol 14 (21): 1929-34.