LumiKine™ kits

Four step bioluminescent ELISA kits

LumiKine™ kits are bioluminescent ELISA kits designed to quantify the levels of cytokines in cell culture supernatant, serum and plasma samples using the classical “sandwich” ELISA method.

LumiKine™  kits include a capture antibody, a biotinylated secondary antibody and a streptavidin-Lucia conjugate for detection. This conjugate consists of a streptavidin peptide fused to Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties. After addition of QUANTI-Luc™, a solution that contains the Lucia luciferase substrate,a bioluminescent signal is  instantaneously emitted. This signal is directly proportional to the concentration of the cytokine produced and quantifiable by a luminometer. The streptavidin-Lucia conjugate can be replaced by the streptavidin conjugate of your choice.

Contents: capture antibody, biotinylated antibody, recombinant cytokine, strepatividin-Lucia, QUANTI-Luc™, white immunoplates

Type I Interferons

LumiKine™ hIFN-αHuman IFN-alpha (multiple subtypes) bioluminescent ELISA kit5 plates
LumiKine™ mIFN-αMouse IFN-alpha (multiple subtypes) bioluminescent ELISA kit5 plates
LumiKine™ hIFN-βHuman IFN-beta bioluminescent ELISA kit5 plates
LumiKine™ mIFN-βMouse IFN-beta bioluminescent ELISA kit5 plates

Interleukin-1

LumiKine™ mIL-1αMouse interleukin 1 alpha bioluminescent ELISA5 plates
LumiKine™ hIL-1βHuman IL1-beta bioluminescent ELISA kit5 plates
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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