Lucia luciferase Reporter Cell Lines

InvivoGen has developed new reporter cell lines that feature the Lucia luciferase reporter gene.

These cell lines allow the study of one or two signal transduction pathways following their activation by diverse molecules such as microbial ligands or cytokines.
They were obtained by stable tranfection of the extensively used human embryonic kidney (HEK) 293 cell line or well-established immune cell models, such as the human monocytic THP-1 cell line.

Some of the cell lines express a dual reporter system containing the Lucia (secreted luciferase) and SEAP (secreted embryonic alkaline phosphatase) reporter genes driven by either the same or two different inducible promoter constructs. Both reporters are readily measurable in the cell culture supernatant when using QUANTI-Luc™, a luciferase detection reagent, and QUANTI-Blue™, a SEAP detection reagent.

HEK-Dual™ TNF-α CellsHuman and Murine TNF-α SEAP / luciferase reporter HEK293 cells - NF-κB pathway3-7 x 10e6 cells
HEK-Dual™ IFN-γ cellsHuman IFN-γ SEAP/Luciferase reporter HEK293 cells
JAK/STAT1/IRF pathway
3-7 x 10e6 cells
THP1-Dual™ CellsHuman THP-1 Monocytes - NF-κB-SEAP IRF-Luc Reporter3-7 x 10e6 cells
THP1-Dual™ KO-MyD CellsHuman THP-1 Monocytes - MyD88 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells3-7 x 10e6 cells
THP1-Lucia™ ISG CellsHuman THP-1 Monocytes - IRF Luciferase Reporter Cells3-7 x 10e6 cells
THP1-Lucia™ NF-κB CellsHuman THP-1 Monocytes - NF-κB Luc Reporter Monocytes3-7 x 10e6 cells
J774-Dual™ CellsMurine NF-κB & IRF Reporter macrophage-like cells3-7 x 10e6 cells
RAW-Lucia™ ISG CellsIRF-Lucia Luciferase Reporter Cell Line3-7 x 10e6 cells
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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