Interferon Response

The RNAi reagents, siRNAs and shRNAs, can be potent inducers of the interferon response due to their recognition by cellular receptors of foreign RNA and DNA, such as TLR3, TLR7/8, TLR9 and RIG-I/MDA-5. To determine whether a given siRNA or shRNA is immunostimulatory, InvivoGen has developed a set of primers, IFNr qRT-Primers, that detect the expression of human genes involved in the IFN response by real-time quantitative PCR.

The IFN response induced by shRNAs results mainly from the DNA vector that contains non-methylated CpG sequences that are recognized by TLR9. InvivoGen has designed CpG-free vectors, pCpGfree-siRNA and pCpG-free-siRNADUO, to overcome this problem.

Interferon Response Products

IFN response Detection
IFNr qRT-Primers RT-PCR primers for the real-time quantification of the IFN response 1 kit

pCpGfree Vectors
pCpGfree-siRNA Expression vector CpG-free for siRNA production in vivo 1 kit
pCpGfree-siRNADUO Expression vector CpG-free to generate simultaneously two shRNAs in vivo 1 kit
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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