Response of HEK-Blue hTLR9 cells to Class B CpG ODNs

Response of HEK-Blue™ hTLR9 cells to Class B CpG ODNs.
HEK-Blue hTLR9™ cells, which express human TLR9 and an NF-κB-inducible SEAP reporter gene, were incubated in HEK-Blue™ Detection medium and stimulated with increasing concentrations of B-class CpG ODNs, ODN 1668, ODN 1826, ODN 2006 or ODN 2007, and their corresponding GC control ODNs.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.