Response of HEK-Blue hTLR9 cells to Class A CpG ODNs

Response of HEK-Blue™ hTLR9 cells to Class A CpG ODNs.
HEK-Blue™ hTLR9 cells, which express human TLR9 and an NF-κB-inducible SEAP reporter gene, were incubated in HEK-Blue Detection medium and stimulated with increasing concentrations of A-class CpG ODNs, ODN 1585, ODN 2216 or ODN 2236, and their corresponding GC control ODNs.
After 24h incubation, the levels of NF-ΚB-induced SEAP were determined by reading the OD at 655 nm.