HKSC

Heat-killed S. cerevisiae

HKSC is a heat-killed preparation of Saccharomyces cerevisiae. HKSC activates the β-glucan specific dectin-1 receptor, which is expressed on phagocytes. β-Glucans are glucose polymers found in the cell walls of fungi. Dectin-1 binds and internalizes β-glucans and mediates the activation of NF-κB and subsequent secretion of proinflammatory cytokines.

Specifications

Specificity: Dectin-1 agonist

Working concentration: 108 cells/ml

Endotoxin level: EndoFit™ (<0.001 EU/μg)

Contents

• 109 freeze-dried cells of heat-killed preparation of Saccharomyces cerevisiae (HKSC)
• 1 ml sterile endotoxin-free water

Description

HKSC is a heat-killed preparation of S.cerevisiae. HKSC activates the β-glucan specific dectin-1 receptor, which is expressed on phagocytes [1]. β-Glucans are glucose polymers found in the cell walls of fungi, such as zymosan (a cell wall preparation of Saccharomyces cerevisiae) and Candida albicans. Dectin-1 binds and internalizes β-glucans and mediates the production of reactive oxygen species (ROS), activation of NF-κB and subsequent secretion of proinflammatory cytokines. However, it is now clear that its β-glucan moiety triggers NF-κB activation only through Dectin-1 as treatment with hot alkali or organic solvents abrogates the TLR2-dependent response [2,3]. RAW-Blue™ cells express high levels of endogenous Dectin-1 and therefore can be used as a Dectin-1 reporter cell line. Stimulation of RAW-Blue™ cells with depleted zymosan or heat-killed preparations of yeast induces the activation of NF-κB in a Dectin-dependent manner. NF-κB activation can be readily monitored as RAW-Blue™ cells stably express an NF-κB-inducible SEAP reporter gene.

1. Brown GD. et al., 2003. Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 197: 1119- 24.
2. Gantner BN. et al., 2003. Collaborative induction of inflammatory respons- es by dectin-1 and Toll- likereceptor 2. J Exp Med. 197: 1107- 17.
3. ikeda Y. et al., 2008. Dissociation of Toll- like receptor 2-mediated innate immune response to Zymosan by organic solvent-treatment without loss of Dectin-1 reactivity.Biol Pharm Bull.31: 13-8.

Citations

Recent articles using InvivoGen HKSC

ORDERING

HKSC

Description Heat-killed S. cerevisiae
Cat. Code tlrl-hksc
Unit Size 10e9 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purposes only. Not for use in humans. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".