HEK-Dual™ mTLR4 (NF/IL8) cells were generated from HEK-Dual™ Null cells by stable transfection of the mouse TLR4 (mTLR4), MD2 and CD14 genes. Due to the knockout of TLR3 and TLR5, these cells enable the study of mTLR4 signaling without interference from other TLRs. They respond to very low concentrations of TLR4 agonists such as lipopolysaccharide (LPS). They do not respond to other TLR agonists or to the cytokine TNF-α (see validation sheet).

HEK-Dual™ mTLR4 (NF/IL8) cells are resistant to hygromycin B, Zeocin™ and blasticidin. They should be maintained in growth medium supplemented with hygromycin B and Zeocin™.

Figures for this product

NF-kB (SEAP) responseKI-IL-8 (Lucia) response


Antibiotic resistance: blasticidinhygromycinZeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


- 1 vial containing 3-7 x 106 cells.
- 100 μl Hygromycin B Gold (100 mg/ml).
- 100 μl Zeocin™ (100mg/ml).
- 1 ml Normocin™ (50 mg/ml).
- 1 pouch of QUANTI-Blue™ (SEAP detection medium).
- 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium).


EC50 values calculated for the NF-kB response using QUANTI-BlueHEK-Dual™ mTLR4 (NF/IL8) and HEK-Blue™ mTLR4 cells were stimulated for 24 hours with various TLR4 agonists: LPS-EB UP, LPS-EK UP and MPLAs.

Cell line EC50 for LPS-EB UP (ng/ml) EC50 for LPS-EK UP (ng/ml) EC50 for MPLAs (ng/ml)
HEK-Dual™ mTLR4 (NF/IL8) 0.06 ± 0.01 1.38 ± 0.35 0.12 ± 0.02
HEK‑Blue™ mTLR4 0.06 ± 0.01 1.21 ± 0.36 0.09 ± 0.02


  1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
  2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38. 


HEK-Dual™ mTLR4 (NF/IL8)NEW!

Description Double readout TLR cells
Cat. Codehkd-mtlr4ni
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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