HEK-Dual™ hTLR5 (NF/IL8) cells were generated from HEK-Dual™ Null cells by stable transfection of the human TLR5 (hTLR5) gene. Due to the knockout of TLR3, these cells enable the study of hTLR5 signaling without interference from other TLRs. They respond to low concentrations of TLR5 agonist flagellin. They do not respond to other TLR agonists or to the cytokine TNF-α (see validation sheet).

HEK-Dual™ hTLR5 (NF/IL8) cells are resistant to hygromycin B, Zeocin™ and blasticidin. They should be maintained in growth medium supplemented with hygromycin B and Zeocin™.

Figures for this product

NF-kB (SEAP) responseKI-IL-8 (Lucia) response


Antibiotic resistance: blasticidinhygromycinZeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


- 1 vial containing 3-7 x 106 cells.
- 100 μl Hygromycin B Gold (100 mg/ml).
- 100 μl Zeocin™ (100mg/ml).
- 1 ml Normocin™ (50 mg/ml).
- 1 pouch of QUANTI-Blue™ (SEAP detection medium).
- 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium).


EC50 values calculated for the NF-kB response using QUANTI-BlueHEK-Dual™ hTLR5 (NF/IL8) and HEK-Blue™ hTLR5 cells were stimulated for 24 hours with various TLR5 agonists: FLA-ST, recFLA-ST and FLA-BS.

Cell line EC50 for FLA-ST (ng/ml) EC50 for recFLA-ST (ng/ml) EC50 for FLA-BS (ng/ml)
HEK-Dual™ hTLR5 (NF/IL8) 1.4 ± 0.7 200 ± 37.1 568.9 ± 195.5
HEK‑Blue™ hTLR5 2.2 ± 1.7 219.4 ± 95.8 410.5 ± 65.6


  1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
  2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38. 


HEK-Dual™ hTLR5 (NF/IL8)NEW!

Description Double readout TLR cells
Cat. Codehkd-htlr5ni
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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