HEK-Dual™ hTLR2 (NF/IL8) cells were generated from HEK-Dual™ Null cells by stable transfection of the human TLR2 (hTLR2) and CD14 genes. Due to the knockout of TLR3 and TLR5, these cells enable the study of hTLR2 signaling without interference from other TLRs. They respond to very low concentrations of TLR2 agonists such as the synthetic lipopeptide Pam3CSK4. They do not respond to other TLR agonists or to the cytokine TNF-α (see validation sheet).

HEK-Dual™ hTLR2 (NF/IL8) cells are resistant to hygromycin B, Zeocin™ and blasticidin. They should be maintained in growth medium supplemented with hygromycin B and Zeocin™.

Figures for this product

NF-kB (SEAP) responseKI-IL-8 (Lucia) response


Antibiotic resistance: blasticidinhygromycinZeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


- 1 vial containing 3-7 x 106 cells.
- 100 μl Hygromycin B Gold (100 mg/ml).
- 100 μl Zeocin™ (100mg/ml).
- 1 ml Normocin™ (50 mg/ml).
- 1 pouch of QUANTI-Blue™ (SEAP detection medium).
- 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium).


EC50 values calculated for the NF-kB response using QUANTI-Blue. HEK-DualhTLR2 (NF/IL8) and HEK-BluehTLR2 cells were stimulated for 24 hours with various TLR2 agonists: FSL-1, Pam3CSK4 and HKLM. 

Cell line EC50 for FSL-1 (pg/ml) EC50 for Pam3CSK4 (pg/ml) EC50 for HKLM (cells/ml)
HEK-Dual™ hTLR2 (NF/IL8) 0.3 ± 0.1 2.5 ± 0.1 0.5 x106 ± 0.1 x106
HEK‑Blue™ hTLR2 2.9 ± 0.5 26.4 ± 2.9 1.6 x106 ± 0.5 x106


  1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
  2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38. 


HEK-Dual™ hTLR2 (NF/IL8)NEW!

Description Double readout TLR cells
Cat. Codehkd-htlr2ni
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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