HEK TLR Reporter Cells

InvivoGen offers two series of HEK293-derived TLR (Toll-like receptor) reporter cells designed to provide a rapid, sensitive and reliable method to screen and validate TLR agonists or antagonists. Both series allow the study of TLR stimulation by monitoring the activation of the transcription factors NF-κB and AP-1.

HEK-Blue™ TLR cells

Well-established family of TLR reporter cells

HEK-Blue™ TLR cells allow the study of TLR stimulation by monitoring the activation of the transcription factors NF-κB and AP-1. They stably co-express a TLR gene and an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene.

 

NEW

HEK-Dual™ TLR (NF/IL8) cells

New family of TLR reporter cells

In addition to the monitoring of TLR-induced NF-κB/AP-1 activation, HEK-Dual™ TLR (NF/IL8) cells allow the monitoring of TLR-induced IL-8 expression. They stably co-express a TLR gene, an NF-κB/AP1-inducible SEAP reporter gene, and the Lucia Luciferase reporter gene placed under the control of the endogenous IL-8 promoter (through the use of knockin technology). Furthermore, these cells have been engineered to eliminate the endogenous activity of TLR3, TLR5 and TNFR (Tumor Necrosis Factor receptor).

The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents, QUANTI-Blue™ or HEK-Blue™ Detection, and the Lucia detection reagent, QUANTI-Luc™.

HEK-Blue™ (NFkB-SEAP) TLR cells

HEK-Blue™ hMD2-CD14Human MD2-CD14 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ hTLR2Human TLR2 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR2Murine TLR2 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ hTLR3Human TLR3 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR3Murine TLR3 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ hTLR4Human TLR4 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR4Murine TLR4 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ hTLR5Human TLR5 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR5Murine TLR5 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ hTLR7Human TLR7 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR7Murine TLR7 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ hTLR8HumanTLR8 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR8Murine TLR8 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ hTLR9Human TLR9 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR9Murine TLR9 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR13Murine TLR13 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells

HEK-DUAL™ (NFKB-SEAP / KI-[IL-8]Lucia) TLR CELLS

HEK-Dual™ hTLR2 NEWHuman TLR2 (NFkB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells3-7 x 10e6 cells
HEK-Dual™ hTLR3 NEWHuman TLR3 (NFkB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells3-7 x 10e6 cells
HEK-Dual™ mTLR4 NEWMurine TLR4 (NFkB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells3-7 x 10e6 cells
HEK-Dual™ hTLR5 NEWHuman TLR5 (NFkB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells3-7 x 10e6 cells
HEK-Dual™ mTLR7 NEWMurine TLR7 (NFkB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells3-7 x 10e6 cells
HEK-Dual™ hTLR9 NEWHuman TLR9 (NFkB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells3-7 x 10e6 cells
HEK-Dual™ Null NEWNFkB-SEAP / KI-[IL-8]Lucia dual-reporter HEK293 cells3-7 x 10e6 cells
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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