HEK-Blue™ mTLR7

HEK-Blue™-mTLR7 cells were obtained by co-transfection of the murine TLR7 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR7 ligand activates NF-κB and AP-1 which induces the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ mTLR7 cells is HEK-Blue™ Null2-k cells.


Antibiotic resistance: blasticidin, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl Blasticidin (10 mg/ml)

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)


HEK-Blue™ mTLR7 cells

Description murine TLR7-expressing HEK 293 cells
Cat. Codehkb-mtlr7
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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