Frequently Asked Questions (FAQ)

  1. Can I use blasticidin and puromycin selection at the same time ?


Can I use blasticidin and puromycin selection at the same time ?

Yes. You may apply both selective agents at the same time without adverse effect. Thus you can transfect cell lines with a blasticin-resistance vector, while maintaining a puromycin selective pressure (to select for a previously introduced puromycin-resistant vector). It works also the other way around (blasticidin resistance vector first), or concomitantly (co-transfection of two vectors).

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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.

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