Blasticidin S is a peptidyl nucleoside antibiotic active on both prokaryotes and eukaryotes. Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr). Selection of common E. coli strains is achieved at 50-100 μg/ml of blasticidin in Low Salt LB medium adjusted to pH 8.
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Description
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It specifically inhibits protein synthesis in both prokaryotes and eukaryotes by interfering with the peptide bound formation in the ribosomal machinery. Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr) which codes for blasticidin deaminase.
Selection of common E. coli strains is achieved at 50-100 μg/ml of blasticidin in Low Salt LB medium adjusted to pH 8.
Contents and Storage
Blasticidin is provided as a colorless solution at 10 mg/ml. Blasticidin is shipped at room temperature and should be stored at -20°C. Blasticidin is stable up to 1 year when stored at -20°
Hygromycin B is an aminoglycoside antibiotic effective on most bacteria, fungi and higher eukaryotes. Resistance to hygromycin is conferred by the hph gene from E. coli. Hygromycin is normally used at a concentration of 50 μg/ml in Low Salt LB Agar adjusted to pH 8.
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Description
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. Hygromycin B is effective on most bacteria, fungi and higher eukaryotes. Resistance to hygromycin is conferred by the hph gene from E. coli.
Hygromycin is normally used at a concentration of 50 μg/ml in Low Salt LB Agar adjusted to pH 8.
Contents and Storage
Hygromycin B is provided as 100 mg/ml yellow solution. Product is shipped at room temperature. Store at -20°C upon receipt. Hygromycin B solutions are stable for at least one year when stored at -20°C.
Zeocin™ is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic effectivel for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Resistance to Zeocin™ is conferred by the Sh ble gene. Zeocin™ is used at a concentration of 25 μg/ml in Low Salt LB medium at pH 7.5.
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Description
Zeocin™ is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it. This antibiotic is effective on most aerobic cells and is therefore useful for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Resistance to Zeocin™ is conferred by the Sh ble gene product which inactivates Zeocin™ by binding to the antibiotic.
Zeocin™ is used at a concentration of 25 μg/ml in Low Salt LB medium at pH 7.5.
Contents and Storage
Zeocin™ is provided as a blue solution at 100 mg/ml. Zeocin™ is shipped at room temperature and upon receipt should be stored at -20°C. Zeocin™ is stable for at least 1 year at -20°C.
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations
and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
