Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. Hygromycin B is effective on most bacteria, fungi and higher eukaryotes. Resistance to hygromycin is conferred by the hph gene from E. coli.
Hygromycin B is normally used at a concentration of 50-200 µg/ml in mammalian cells and 100 µg/ml in bacteria.
Two grades of Hygromycin B are available: Hygromycin B (purity >85%) HygroGold™ (purity >98%)
Formula: C20H37N3O13, HCl Molecular weight: 527.52 CAS Number : 31282-04-9
Bulk quantities available at highly discounted prices: Contact info@invivogen.com
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Cloning vectors using Hygromycin B
Invivogen offers several selection vectors using Hygromycin as selection antibiotic:
- pSELECT-hygro-mcs, pSELECT-hygro-LacZ : pSELECT plasmids offer all the features necessary to express a gene of interest at high levels in a large number of cell types. - pMONO-hygro-mcs, pMONO-hygro-gfp : pMONO plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. - pVITRO1-hygro-mcs, pVITRO1-hygro-GFP/LacZ : pVITRO allow the ubiquitous and constitutive co-expression of two genes of interest. - pVITRO2-hygro-mcs, pVITRO2-hygro-GFP/LacZ : pVIVO allow strong and sustained co-expression of two genes of interest in many tissues and organs. - psiRNA-h7SK G1 Hygro : psiRNA-h7SK offer all the features necessary to express shRNA under the human 7SK RNA polymerase III promoter. - pCpGfree-vitroHmcs, pCpGfree-vitroHLacZ : pCpGfree-vitro is a family of expression vectors completely devoid of CpG dinucleotides that are selectable in mammalian cells
Contents and Storage
Hygromycin B and HygroGold™ are provided as 100 mg/ml yellow solutions. HygroGold™ is also provided as a powder. Products are shipped at room temperature. Store at -20°C. Hygromycin B solutions are stable for at least one year when stored at -20°C.
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations
and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. Hygromycin B is effective on most bacteria, fungi and higher eukaryotes. 
