G418 is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the neo gene from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘ II.
Selection in mammalian cells is usually achieved in three to seven days with concentrations ranging from 400 to 1000 µg/ml. Cells that are dividing are affected sooner than those that are not.
Formula: C20H40N4O10. 2H2SO4 Molecular weight: 692.7 CAS Number: 108321-42-2
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Cloning vectors using G418
- pSELECT-neo-mcs, pSELECT-neo-LacZ : pSELECT plasmids offer all the features necessary to express a gene of interest at high levels in a large number of cell types. - pMONO-neo-mcs, pMONO-neo-gfp : pMONO plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. - pVITRO1-neo-mcs, pVITRO1-neo-GFP/LacZ : pVITRO allow the ubiquitous and constitutive co-expression of two genes of interest. - pVITRO2-neo-mcs, pVITRO2-neo-GFP/LacZ : pVIVO allow strong and sustained co-expression of two genes of interest in many tissues and organs. - psiRNA-h7SK G1 Neo : psiRNA-h7SK offer all the features necessary to express shRNA under the human 7SK RNA polymerase III promoter. - pCpGfree-vitroNmcs, pCpGfree-vItroNLacZ : pCpGfree-vitro is a family of expression vectors completely devoid of CpG dinucleotides that are selectable in mammalian cells
Contents and Storage
G418 is provided as a colorless solution at 100 mg/ml. G418 is shipped at room temperature and should be stored at -20°C. G418 is stable for at least 1 year when stored at -20°C.
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations
and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
G418 is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. 
