Our Products
pUNO-HA
HA-tagged TLR and IRF Genes
Features and Benefits
Convenient and Efficient Detection of TLRs & IRFs
pUNO-HA is a family of expression vectors featuring HA-tagged TLR and IRF (Interferon Regulatory Factor) genes. The genes have been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged TLR genes provides a simple and convenient method to detect the expression of the TLR genes by Western blot. All TLR genes can be detected using the same primary antibody, the HA tag monoclonal antibody.
Easy Selection of TLR-HA and IRF-HA Clones
pUNO-HA plasmids carry a blasticidin resistance gene for amplification in E. coli and selection in mammalian cells.
Blasticidin is a potent antibiotic that allows the selection of stable mammalian clones in as little as one week.
Contents and Storage
Each pUNO-HA plasmid is provided as a lyophilized transformed E. coli strain on a paper disk. Transformed strains are shipped at room temperature and should be stored at -20°C
Lyophilized E. coli cells are stable for at least 1 year when properly stored.
Each plasmid is provided with 4 pouches of E.coli Fast-Media®
Blas (2 TB and 2 Agar).
pUNO Control
| Product | Qty | Cat. Code | Price | Order |
|---|---|---|---|---|
| pUNO-mcs | 1 disk | puno-mcs | check |  |
| pUNO-mcs purchased with a pUNO-[gene] | 1 disk | puno-mcs. | check | |
| pUNO1-mcs | 1 disk | puno1-mcs | check | |
| pUNO1-mcs purchased with a pUNO-[gene] | 1 disk | puno1-mcs. | check | |
Anti-HA Antibody
The monoclonal antibody Anti-HAtag recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The specificity of this antibody allows for unambiguous identification of the tagged protein. Anti-HAtag can be used for detection of HA-tagged proteins by Western Blot or immunoprecipitation.
| Product | TDS | MSDS | Qty | Cat. Code | Price | Order |
|---|---|---|---|---|---|---|
| Anti-HA Tag | 250 µl | ab-hatag | check | |
