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pUNO
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Plasmid Expressing One TLR or TLR-Related Gene
Toll-like receptors interact with co-receptor molecules to recognize specific molecular patterns associated with microbial pathogens. Upon recognition, they induce distinct cellular responses that involve different adaptor proteins which in turn will activate a cascade of signaling genes leading to the production of proinflammatory cytokines. InvivoGen offers a large selection of human and mouse genes involved in the TLR pathways.
Description
Fully Active Genes
All genes cloned correspond to the open reading frame or coding sequence, from the ATG to the Stop codon. The genes have been fully sequenced, their expression verified by RT-PCR and their function tested in HEK293 cells coexpressing an NF-κB reporter plasmid.
Ready-to-use Vectors
pUNO plasmids can be used directly for in vitro or in vivo transfection experiments. They are selectable with blasticidin, an antibiotic that allows the selection of stable mammalian clones in only a few days.
High Levels of Expression
TLR/TLR-related genes are under the control of a strong and ubiquitous EF1a/HTLV composite promoter.
Convenient Subcloning of the TLR Genes
To facilitate the excision and subcloning of a TLR/TLR-related gene into another vector, each gene is flanked by unique restriction sites that are compatible with many others.
Contents
Each pUNO plasmid is provided as a lyophilized transformed E. coli strain on a paper disk. Transformed strains are shipped at room temperature and should be stored at -20°C. Lyophilized E. coli cells are stable for at least 1 year when properly stored.
Each plasmid is provided with 4 pouches of E. coli Fast-Media© Blas (2 TB and 2 Agar).
| Product | Qty | Cat. Code | Price | Order |
|---|---|---|---|---|
| pUNO-mcs | 1 disk | puno-mcs | check |  |
| pUNO-mcs purchased with a pUNO-[gene] | 1 disk | puno-mcs. | check | |
| pUNO1-mcs | 1 disk | puno1-mcs | check | |
| pUNO1-mcs purchased with a pUNO-[gene] | 1 disk | puno1-mcs. | check | |