Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It specifically inhibits protein synthesis in both prokaryotes and eukaryotes by interfering with the peptide bound formation in the ribosomal machinery. Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr) which codes for blasticidin deaminase.
Typically, bacteria are sensitive to blasticidin concentrations of 25-100 µg/ml, and mammalian cells to 1-10 µg/ml.
Formula: C17H26N8O5, 1 HCl Molecular weight: 458.5 CAS Number: 2079-00-7
Bulk quantities available at highly discounted prices: Contact info@invivogen.com
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Cloning vectors using Blasticidin S
Invivogen offers several selection vectors using Blasticidin S as selection antibiotic:
- pSELECT-blasti-mcs, pSELECT-blasti-LacZ : pSELECT plasmids offer all the features necessary to express a gene of interest at high levels in a large number of cell types. - pMONO-blasti-mcs, pMONO-blasti-gfp : pMONO plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. - pVITRO1-blasti-mcs, pVITRO1-blasti-GFP/LacZ : pVITRO allow the ubiquitous and constitutive co-expression of two genes of interest. - pVITRO2-blasti-mcs, pVITRO2-blasti-GFP/LacZ : pVIVO allow strong and sustained co-expression of two genes of interest in many tissues and organs. - psiRNA-h7SK G1 Blasti : psiRNA-h7SK offer all the features necessary to express shRNA under the human 7SK RNA polymerase III promoter. - pCpGfree-vitroBLacZ : pCpGfree-vitro is a family of expression vectors completely devoid of CpG dinucleotides that are selectable in mammalian cells
Contents
Blasticidin is provided as a colorless solution at 10 mg/ml. Blasticidin is shipped at room temperature and should be stored at -20°C. Blasticidin is stable up to 1 year when stored at -20°C.
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations
and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It specifically inhibits protein synthesis in both prokaryotes and eukaryotes by interfering with the peptide bound formation in the ribosomal machinery. 