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Blues™ Reporter Cells

butterflybutterflyBlue™ Reporter Cells is a family of engineered cell lines designed to provide a simple, rapid and reliable method to monitor the activation of intracellular signaling pathways induced by microbial molecules or cytokines.

This method is based on the inducible expression of the secreted embryonic alkaline phosphatase (SEAP) reporter gene. Exposure to a stimulus induces the pathway modulating the SEAP activity that can be readily assessed qualitatively and quantitatively using QUANTI-Blue™. This SEAP detection medium turns blue in the presence of the reporter protein.

Check the Blue™ cells Validation Sheets.

Blue™ family

Pattern Recognition Receptor Reporter Cells
HEK-Blue™ TLR/NOD reporter cells : human HEK 293
CH3 MEF: murine embryonic fibroblasts
C57 MEF: murine embryonic fibroblasts
B16-Blue™: murine B16 melanoma

Immune Reporter Cells
THP1-XBlue™: human monocytic THP-1
Ramos-Blue™: human B lymphocyte
RAW-Blue™: murine macrophage

Cytokine Reporter Cells
B16-Blue™: murine B16 melanoma
HEK-Blue™ : human HEK 293

 

 

Pattern Recognition Receptor Reporter Cells

Reporter Gene activation of TLRs, NODs and RIG-I/MDA-5 in InvivoGen's Reporter cell lines

Reporter cell line   Cell line Reporter system TLR1 TLR2 TLR3 TLR4 TLR5 TLR6 TLR7 TLR8 TLR9 NOD1 NOD2 RIG-I/MDA-5
HEK-Blue™ hTLR2 human HEK 293 NF-κB/AP-1 SEAP E ++ E - E E - - - E - ND
HEK-Blue™ hTLR3 human HEK 293 NF-κB/AP-1 SEAP E - ++ - E E - - - E - ND
HEK-Blue™ hTLR4 human HEK 293 NF-κB/AP-1 SEAP E - E ++ E E - - - E - ND
HEK-Blue™ hTLR5 human HEK 293 NF-κB/AP-1 SEAP E - E - ++ E - - - E - ND
HEK-Blue™ hTLR7 human HEK 293 NF-κB/AP-1 SEAP E - E - E E ++ - - E - ND
HEK-Blue™ hTLR8 human HEK 293 NF-κB/AP-1 SEAP E - E - E E - ++ - E - ND
HEK-Blue™ hTLR9 human HEK 293 NF-κB/AP-1 SEAP E - E - E E - - ++ E - ND
HEK-Blue™ hNOD1 human HEK 293 NF-κB/AP-1 SEAP E - E - E E - - - ++ - ND
HEK-Blue™ hNOD2 human HEK 293 NF-κB/AP-1 SEAP E - E - E E - - - E ++ ND
HEK-Blue™ Null1 human HEK 293 NF-κB/AP-1 SEAP E - E - E E - - - E - ND
HEK-Blue™ Null2 human HEK 293 NF-κB/AP-1 SEAP E - E - E E - - - E - ND
C3H/WT MEF murine Murine embryonic fibroblasts NF-κB/AP-1 SEAP + + +/- + +/- + - ND - ND ND +
C3H/TLR4mut MEF murine Murine embryonic fibroblasts NF-κB/AP-1 SEAP + + +/- - +/- +
 - ND - ND ND +
C57/WT MEF murine
 Murine embryonic fibroblasts NF-κB/ISRE SEAP + + -
 + +/- +
 - ND - + - +
B16-Blue™ IFNα/β  murine B16 melanoma
ISRE SEAP
 -
 -  +/-  - -
-
 -  - -
 - -
++

E: endogenous expression,  ++: overexpression / strongly induced, +/-: lightly induced, - : no expression, ND: non described

Immune Reporter Cells

Reporter Gene activation of TLRs, NODs, RIG-I/ MDA-5 in InvivoGen's Reporter Cell lines

Reporter cell lines   Cell line Reporter system TLR1 TLR2 TLR3 TLR4 TLR5 TLR6 TLR7 TLR8 TLR9 NOD1 NOD2 RIG-I/MDA-5
THP1-XBlue human monocytic THP-1 NF-κB/AP-1 SEAP ++ ++ -
 +
 +/- ++ - ++
 - + +
 ND
THP1-XBlue-CD14 human monocytic THP-1 NF-κB/AP-1 SEAP ++ ++ - + +/- ++ - ++ - + +
 ND
THP1-XBlue-defMyD  human monocytic THP-1 NF-κB/AP-1 SEAP -
-
-
-
-
-
 -
-
-
+
+
ND
Ramos-Blue human B lymphocyte NF-κB/AP-1 SEAP +/- +/- ++ - - +/- ++ - + + - ND
RAW-Blue murine macrophage NF-κB/AP-1 SEAP ++ ++ +/- ++ - ++ + ND + +/- + ND

++: strongly induced, +: induced, +/-: lightly induced, -: non induced, ND: non described

Cytokine Reporter Cells

Cytokines Detected by cytokine reporter cells

Cytokine Sensor Cells   Cell line Reporter system IFNα IFNβ IFNγ IL-1β IL-4 IL-6 IL-13 IL-18 TGF-β TNFα CD40L
B16-Blue™ IFN-α/β cells murine B16 melanoma ISRE SEAP  ++ ++ - - - - - - - - -
HEK-Blue™ IFN-α/β cells human HEK 293 ISRE SEAP  ++ ++
-
-
-
-
-
-
-
-
-
HEK-Blue™ IL-4/IL-13 cells human
HEK 293 STAT6 SEAP +/-
 -
 -
 -
 ++
 -
 ++
 -
 -
 -
 -
HEK-Blue™ IL-6 cells human HEK 293 STAT3 SEAP  - -
 -
 -
 -
 ++
 -
 -
 -
 -
 -
HEK-Blue™ TGF-β cells human HEK 293 SBE SEAP -
 -
 -
 -
 -
 -
 -
 -
 ++
 -
 -
HEK-Blue™ TNF-α/IL-1β cells human
HEK 293 NF-κB/AP-1 SEAP - - - ++ - - -   - ++ -
HEK-Blue™ IL-1β cells human
HEK 293 NF-κB/AP-1 SEAP - - - ++ - - - - - - -
HEK-Blue™ IL-18/IL-1β cells human HEK 293 NF-κB/AP-1 SEAP - - - ++ - - - ++ - - -
HEK-Blue™ CD40L cells human
HEK 293 NF-κB/AP-1 SEAP -
 -
 -
 ++
 -
 -
 -
 -
 -
 ++
 ++

++: strongly induced, +: induced, +/-: lightly induced, -: non induced

Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.

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