Our Products
- TLR8 ligands
- TLR7 ligands
- TLR7/8 ligands
- Toll-Like Receptor 8
- TLR8 Expressing Cell lines
TLR8 Ligands
TLR8 play an important role in the immune response to viral infection. It recognize single stranded RNAs as its natural ligand and also small synthetic molecules.
TLR8 Ligands (human TLR8 & mouse TLR7)
- Single-Stranded RNAs
- E.coli RNA
TLR7/8 Ligands (human, mouse TLR7 & human TLR8)
- CL075: thiazoloquinoline compound
- CL097: water-soluble R848, imidazoquinoline compound
- Poly(dT): thymidine homopolymer phosphorothioate ODN
- R848: Imidazoquinoline compound
TLR8 ligands
Single-Stranded RNAs
Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1, 2]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC. This induction was reproduced using polyU or GU-rich (ssRNA40) ODNs complexed with cationic lipids to protect them from degradation. Upon stimulation with ssRNA, murine TLR7 and human TLR8 induced the activation of NF-κB, whereas human TLR7 and murine TLR8 failed, implying a species specificity difference in ssRNA recognition.
1. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 5;303(5663):1529-31
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
Contents and Storage
ssRNAs/LyoVec and ORNs/LyoVec are complexed with the transfection reagent LyoVec to protect them from degradation and facilitate their cellular uptake.They are provided as 4 vials of 25 µg lyophilized complexes.
Poly(U) is also provided alone in two quantities: 10 mg and 100 mg. Poly(U) is provided lyophilized.
All ssRNAs are shipped at room temperature. Store at -20°C. Lyophilized product is stable 1 year at -20˚C.
| Product | Description | Endotoxin Level | Working Conc. | TDS | MSDS | Qty | Cat. Code | Price | Order |
|---|---|---|---|---|---|---|---|---|---|
|
ORN 02 / LyoVec
|
Endotoxin-free | 0.25 - 5 µg/ml | 100 µg | tlrl-orn2 | check |  | |||
|
ORN 06 / LyoVec
|
Endotoxin-free | 0.25 - 5 µg/ml | 100 µg | tlrl-orn6 | check | | |||
|
ssPolyU / LyoVec
|
Endotoxin-free | 1 - 10 µg/ml | 100 µg | tlrl-lpu | check | | |||
|
ssRNA40 / LyoVec
|
Endotoxin-free | 0.25 - 5 µg/ml | 100 µg | tlrl-lrna40 | check | | |||
|
ssRNA41 / LyoVec
|
ssRNA40 control | Endotoxin-free | 0.25 - 5 µg/ml | 100 µg | tlrl-lrna41 | check | | ||
|
ssRNA-DR / LyoVec
|
Endotoxin-free | 1 - 10 µg/ml | 100 µg | tlrl-ssdr | check | | |||
|
Poly(U)
NEW
|
< 5 EU/mg | 10 mg | tlrl-sspu | check | | ||||
|
Poly(U)
NEW
|
< 5 EU/mg | 100 mg (10 x 10 mg) | tlrl-sspu-100 | check | |
E.coli RNA
Total RNA derived from bacteria, but not from eukaryotic cells, induces high levels of IL-12 secretion in dendritic cells[1]. This difference is explained by the fact that bacterial RNA contains significantly less nucleoside modifications than mammalian RNA[2]. Indeed nucleoside modifications have been reported to suppress the immunostimulatory effect of RNA. Bacterial RNA complexed with a cationic lipid (LyoVec™) induce the activation of NF-κB in HEK293 cells expressing human TLR8 or mouse TLR7.
1. Koski GK. et al., 2004. Cutting edge: innate immune system discriminates between RNA containing bacterial versus eukaryotic structural features that prime for high-level IL-12 secretion by dendritic cells.J Immunol. 172(7):3989-93.
2. Kariko K. et al., 2005. Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA. Immunity. 23(2):165-75.
| Product | Description | Endotoxin Level | Working Conc. | TDS | MSDS | Qty | Cat. Code | Price | Order |
|---|---|---|---|---|---|---|---|---|---|
| E. coli RNA / LyoVec | E. coli K12 RNA/LyoVec complexes | Endotoxin-free | 1 - 10 µg/ml | 100 µg | tlrl-ecrna | check | |
