IgA Detection & Quantification

Goat F(ab’)2 anti-human kappa (Capture antibody)
Goat F(ab’)2 anti-human kappa was generated from antibodies isolated from antisera of goats hyperimmunized with human myeloma proteins containing κ light chains. Antibodies were purified by affinity chromatography and digested by pepsin to remove the Fc portion, to avoid non-specific binding through Fc receptors.
Goat F(ab’)2 anti-human kappa allows the capture of κ light chain containing human IgA antibodies.

Goat anti-human kappa - HRP (Revelation antibody)
Goat anti-human kappa-HRP is conjugated with horseradish peroxidase to allow the detection of human IgA kappa light chain by ELISA.

Human IgA kappa (IgA light chain standard)
Human IgA kappa is a human IgAκ isotype control purified from human myeloma serum.

Goat F(ab’)2 anti-human kappa is provided as 0.5 mg of purified immunoglobulin in 1.0 ml of 100 mM borate buffered saline, pH 8.0.
Goat anti-human kappa-HRP is supplied as 1.0 ml of stock solution in 50% glycerol/50% PBS, pH 7.4.
Human IgA kappa is provided filtered sterile at a concentration of 0.5 mg/ml in 1.0 ml of 100 mM borate buffered saline, pH 8.0.
Products are shipped at room temperature and should be stored at 4°C.

Goat F(ab’)2 anti-human IgA (Capture antibody)
Goat F(ab’)2 anti-human IgA was generated from antibodies isolated from antisera of goats hyperimmunized with human IgA paraproteins.
Antibodies were purified by affinity chromatography and digested by pepsin to remove the Fc portion, to avoid non-specific binding through Fc receptors.
Goat F(ab’)2 anti-human IgA allows the capture of the heavy chain of human IgA antibodies.

Goat anti-human IgA - HRP (Revelation antibody)
Goat anti-human IgA-HRP is conjugated with horseradish peroxidase to allow the detection of human IgA heavy chain by ELISA.

Human IgA from colostrum (IgA standard)
Human IgA is isolated from pooled normal human colostrum by fractionation and ion-exchange chromatography.

Goat F(ab’)2 anti-human IgA is provided as 0.5 mg of purified immunoglobulin in 1.0 ml of 100 mM borate buffered saline, pH 8.0.
Goat anti-human IgA-HRP is supplied as 1.0 ml of stock solution in 50% glycerol/50% PBS, pH 7.4.
Human IgA is supplied as an essentially salt-free, lyophilized powder.
Products are shipped at room temperature and should be stored at 4°C.

J chain antiserum was prepared by injection of purified human J chain in rabbits.  This antiserum can be used for the detection of polymeric IgA and pentameric IgM by Western blotting (working dilution 1:1,000).
This anti-human antiserum cross-reacts with the mouse J chain and is reported to cross react with numerous other species [1].

1. Kobayashi K et al. Identification of J-Chain in Polymeric Immunoglobulins from a Variety of Species by Cross-Reaction with Rabbit Antisera to Human J-Chain The Journal of Immunology, 1973, 111, 1590 -1594

J chain antiserum is provided as 100 μg lyophilized antiserum.
J chain antiserum is sterile and azide-free.
Product is shipped at room temperature and should be stored at -20°C or 4°C once resuspended.