Our Products
pUNO-TLR-GFP
TLR-GFP fusion proteins can be used to study the localization of the TLRs. Transfected cells can be analyzed for GFP expression by flow cytometry and by Western-blotting using GFP antibodies.
Features and Benefits
TLR-GFP fusion genes were generated by fusing the GFP gene to the C terminus of various human TLR genes (TLR1 to TLR6). The TLR-GFP fusion genes are cloned in the pUNO plasmid under the control of the strong and ubiquitous mammalian promoter EF1α/HTLV. The pUNO plasmid is selectable with blasticidin in both E. coli and mammalian cells.
All TLR::GFP fusion genes have been fully sequenced, their fluorescence confirmed and their function tested in HEK293 cells coexpressing an NF-κB reporter plasmid and stimulated with the appropriate ligand.
Contents and Storage
Each pUNO-TLR-GFP plasmid is provided as a lyophilized transformed E. coli strain on a paper disk.
Transformed strains are shipped at room temperature and should be stored at -20ºC. Lyophilized E. coli cells are stable for at least 1 year when properly stored.
Each plasmid is provided with 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar,).
| Gene | Species | Product | TDS/ Map | Cat. Code | Price | Order |
|---|---|---|---|---|---|---|
|
TLR1-GFP
|
human | pUNO-hTLR1-GFP | phtlr1-gfp | check |  | |
|
TLR2-GFP
|
human | pUNO-hTLR2-GFP | phtlr2-gfp | check | | |
|
TLR3-GFP
|
human | pUNO-hTLR3-GFP | phtlr3-gfp | check | | |
|
TLR4-GFP
|
human | pUNO-hTLR4-GFP | phtlr4-gfp | check | | |
|
TLR5-GFP
|
human | pUNO-hTLR5-GFP | phtlr5-gfp | check | | |
|
TLR6-GFP
|
human | pUNO-hTLR6-GFP | phtlr6-gfp | check | |