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TLR Ligand Screening
InvivoGen has developed a high-throughput method to determine whether a compound is recognized by Toll-Like Receptors and acts either as an agonist or antagonist. This method is based on TLR-induced activation of various transcription factors including NF-κB, AP1 and IRF7 in HEK293 clones.
Key Service Features
• Short turnaround time - Screening turnaround: ONLY 2 weeks
• Screening flexibility - Screening parameters can be selected and/or modified based on customer requirements. Level 1 and level 2 can be carried out on additional TLRs, including mouse TLRs. More compound concentrations can be tested.
• Cost effective - A set-up charge applies for the first compound. Subsequent compounds are heavily discounted.
Description
Two choices of services are offered, level 1 and level 2, that can be performed sequentially or individually.
Level 1: Single dose testing on a panel of human TLRs Testing is carried out on seven TLRs.
- TLR2: wide array of microbial molecules
- TLR3: dsRNA
- TLR4: Gram-negative lipopolysaccharide
- TLR5: flagellin
- TLR7: single-stranded RNA and small antiviral molecules
- TLR8: single-stranded RNA and small antiviral molecules
- TLR9: specific unmethylated CpG oligonucleotide sequences
Screening is performed at a single concentration, typically a 1/10 dilution of the original compound solution provided, or customer specified.
Duplicate tests (plus controls, see list).
Level 2: Dose response on one or two TLRs Three concentrations of the compound(s), typically 1/10, 1/100 and
1/1000 dilutions of the original compound solution, are tested on the TLR(s) recognizing the compound(s) as determined in level 1 or specified by the customer. Triplicate tests (plus controls).
TLR ligands are typically recognized by a single TLR, potentially two (TLR7 and TLR8). Recognition by TLR4 usually reflects the presence of endotoxins in the sample.
Compounds can be supplied as stock solutions or a solid materials for water-soluble compounds.
A detailed report will be prepared and provided to the customer electronically and in hard copy. All procedures are performed accordingly to strict guidelines. Confidentiality is guaranteed.
Two cytoplasmic proteins involved in pathogen recognition, NOD1 and NOD2, can be added to this screening service. Their activation by a given compound is detected using the same method as the one described above.
| TLR | Ligands | Concentration |
| TLR2 | HKLM | 108 cells/ml |
| TLR3 | Poly(I:C) | 1 μg/ml |
| TLR4 | E.coli K12 LPS | 100 ng/ml |
| TLR5 | S. typhimurium flagellin | 100 ng/ml |
| TLR7 | Imiquimod | 3 μg/ml |
| TLR8 | ssPolyU/LyoVec | 10 μg/ml |
| TLR9 | CpG ODN 2006 | 10 μg/ml |
| NOD1 | Tri-DAP | 1 μg/ml |
| NOD2 | Muramyl dipeptide | 100 ng/ml |
| Product | Cat.Code | Contact Us |
|---|---|---|
| TLR / NOD Ligand Screening | tlrl-test | contact us |