Large Scale Plasmid DNA Production
If you are conducting gene therapy research, DNA vaccination research, transfection or pre-clinical trials, we can provide you with large quantities of plasmid DNA. We offer a comprehensive custom service. In order to supply the most suitable and ready-to-use product, we manufacture plasmid DNA at standard research and pre-clinical grades.
• Technological Expertise
• Personalized Technical Support
• High Quality Plasmid DNA
• Cost-Effective
• Short Turn-Around Time
Two purification grades of plasmid DNA
If you are conducting gene therapy research, DNA vaccination research, transfection or pre-clinical trials, we can provide you with large quantities of plasmid DNA. We offer a comprehensive custom-made service. In order to supply the most suitable and ready-to-use product, we manufacture plasmid DNA at standard research and pre-clinical grades.
- Standard Research Grade
The level of endotoxin is reduced to less than 0.1 EU/μg plasmid DNA (FDA specifications).
- Pre-Clinical Grade
The purification is guaranteed with a protein level less than 1% and with an absence of microorganisms (verified by Bioburden Assay).
High Standard Quality Control
Our processing and our rigorous quality procedures allow us to provide certified plasmid DNA. Each batch of plasmid DNA is delivered with a certificate of analysis and meets the characteristics listed below:
| CRITERIA TESTED | VALUE | ASSAY METHOD |
|---|---|---|
| Appearance in water solution | Clear, colorless | Visual inspection |
| Plasmid identity | Restriction pattern | Agarose gel electrophoresis |
| DNA homogeneity | >95% supercoiled form | Agarose gel electrophoresis |
| Purity | 1.7-2.0 A260/A280 | Optical density measurements |
| Presence of E. coli DNA | <5% | Agarose gel electrophoresis |
| Presence of RNA and ss DNA | Undetectable | Agarose gel electrophoresis |
| Presence of endotoxin | < 0.1 EU / μg plasmid DNA | BioWhittaker LAL test |
| Presence of protein (pre-clinical grade only) | <1% | Micro BCA assay |
| Sterility (pre-clinical grade only) | Absence of microorganisms | Bioburden assay |
| Product | Quantity | Contact Us |
|---|---|---|
| DNA Standard Research Grade | Up to 1 g | contact us |
| DNA Pre-Clinical Grade | Up to 1 g | contact us |
