TLR7/8 agonists dose response in HEK-Blue™ hTLR8 cells

TLR7/8 agonists dose response

HEK-Blue™ hTLR8 cells were incubated in HEK-Blue™ Detection medium and stimulated with increasing concentrations of TLR7 and TLR7/8 agonists. After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm. The response ratio was calculated by dividing the OD at 655 nm for the treated cells by the OD at 655 nm for the untreated cells.


Ligand EC50 Response Ratio
 CL097 N/D
ssRNA40/LyoVec N/D
R848 1 +/- 0.3 µg/ml 8