Figure 1A: IRF INDUCTION (Lucia luciferase reporter)

IRF INDUCTION (Lucia luciferase reporter)

Stimulation of RAW-Lucia™-ISG-KO-MAVS and RAW-Lucia™-ISG cells (wild-type cell line) with VACV70/LyoVec™ (1 µg/ml), poly(dA:dT)/LyoVec™ (1 µg/ml), poly(I:C)/LyoVec™ (1 µg/ml), 5`’ppp-dsRNA/LyoVec™ (1 µg/ml), 2’3’-cGAMP (3 µg/ml) and c-di-AMP (3 µg/ml). Mouse IFN-α (1x104 U/ml) and IFN-β  (1x104 U/ml) serve as positive controls. Non-induced cells (NI) have been included as a negative control. After 24h incubation, IRF activation was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI-Luc™, a Lucia luciferase detection reagent. The IRF induction of each ligand is expressed relative to that of mIFN-β at 1x104 U/ml (taken as 100%).