NF-κB induction in HCT116-Dual™ cells

NF-κ B INDUCTION (SEAP reporter)

Stimulation of HCT116-Dual™ cells with the following PAMPs, Tri-DAP (NOD1 ligand, 10 µg/ml), FLA-ST Ultrapure (TLR5, 1 µg/ml), MDP (10 µg/ml), and Poly(I:C) (TLR3 ligand, 1 µg/ml) leads to the activation of NF-κB. IL-1β (100 ng/ml) has been included as positive controls to activate the NF-κB signaling pathway. Non-induced cells (NI), the TLR2 ligand (Pam3CSK4; 300 ng/ml), and the TLR4 ligand (LPS-EB Ultrapure; 104 EU/ml) have been included as negative controls. After a 24h incubation, NF-κB activation was determined using QUANTI-Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm.