IRF induction in HCT116-Dual™ cells

IRF-INDUCTION (Lucia luciferase reporter)

Stimulation of HCT116-Dual™ cells with the STING agonist 2'3'-cGAMP (30 µg/ml) or RLR ligands, such as transfected poly(I:C) (100 ng/ml) or poly(dA:dT) (100 ng/ml), triggers the IRF pathway. IFN-α (1x104 U/ml) has been included as a positive control to activate the IRF signaling pathway. Non-induced cells (NI), the TLR2 ligand (Pam3CSK4; 300 ng/ml), and the TLR4 ligand (LPS-EB Ultrapure; 104 EU/ml) have been included as negative controls. After a 24h incubation, IRFactivation was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI-Luc™, a Lucia luciferase detection reagent