IRF induction in A549-Dual™ cells

a549 dual irf INDUCTION (Lucia luciferase reporter)

Stimulation of A549-Dual™ cells with RLR ligands, such as transfected poly(I:C) (100 ng/ml), 5'ppp-dsRNA (1 µg/ml) or poly(dA:dT) (100 ng/ml) triggers the IRF pathway. The STING agonist 2'3'-cGAMP (30 µg/ml) leads to low-level IRF induction in A549-Dual™ cells. IFN-α (1x104 U/ml) has been included as a positive control to activate the IRF signaling pathway. Non-induced cells (NI), the TLR4 ligand (LPS-EB Ultrapure; 103 EU/ml), and the TLR7/8 ligand (R848; 10 µg/ml) have been included as negative controls. After a 24h incubation, IRF activation was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI-Luc™, a Lucia luciferase detection reagent.