IRF response of RAW-Lucia™ ISG cells

IRF response of THP1-Lucia™ ISG and RAW-Lucia™ ISG cells

Cells were transfected with CDS ligands (1 µg/ml of HSV-60, HSV-60c, VACV-70, VACV-70c, ISD, ISD Control or 0.3 µg/ml pCpGfree-giant, poly(dA:dT), poly(dA), poly(dG:dC) using LyoVec™ or directly stimulated with 10 µg/ml c-di-AMP or c-di-GMP.
After 24h incubation, the levels of IRF-induced Lucia luciferase were determined using QUANTI-Luc™.