IL-1β production in THP1-null and THP1-defASC cells following their stimulation with ATP and MSU

IL-1beta-production-in-THP1-null-and-THP1-defASC-cells-following-their-stimulation-with-ATP- and-MSU

Cells primed with LPS (1 μg/ml) were stimulated with ATP (5 mM) or MSU (100 μg/ml). After 24h incubation, the supernatants were added to HEK-Blue™ IL-1β cells. IL-1β-induced activation of NF-κB was assessed by measuring the levels of SEAP in the supernatant of HEK-Blue™ IL-1β cells using the QUANTI-Blue™ assay.