NF-κB induction in A549-Dual™ cells

NF-κ B

Stimulation of A549-Dual™ cells with the following PAMPs, Pam3CSK4 (TLR2 ligand, 300 ng/ml) Poly(I:C) (TLR3 ligand, 3 µg/ml ), FLA-ST Ultrapure (TLR5, 300 ng/ml), leads to the activation of NF-κB. TNF-α (1 ng/ml) and IL-1β (1 ng/ml) have been included as positive controls to activate the NF-κB signaling pathway. Non-induced cells (NI), the TLR4 ligand (LPS-EB Ultrapure; 103 EU/ml), and the TLR7/8 ligand (R848; 10 µg/ml) have been included as negative controls. After a 24h incubation, NF-κB activation was determined using QUANTI-Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm.