Custom CpG-Free Genes

Custom CpG-free geneDesign and Synthesis of CpG-Free Genes

InvivoGen is an expert in developing CpG genes and now offers a CpG-free gene custom service. Send us the sequence of your gene of interest, and our specialists will design, synthesize and clone into an expression vector a CpG-free allele of this gene. We guarantee that the sequence of the synthetic gene will 100% match the designed sequence.

Key Service Features

- Free Sequence Design
- Short Turnaround Time
- Competitive Price
- Confidentiality Guaranteed


Sequence Design

Our experts will design the sequence of a CpG-free allele of your gene of interest using a proprietary software that will include the following:
- elimination of all CpG dinucleotides
- humanization of the sequence
- elimination of secondary structures such as hairpins and long direct repeated sequences in order to increase the stability and expression of the mRNA
- elimination/reduction of dam and dcm methylations to diminish E.coli signature

Cloning in an Expression Vector

The Custom-made CpG-free Gene is cloned into the pMA plasmid. We can clone it in the vector of your choice for an additional charge.


The Custom-made CpG-free Gene is 100% sequence verified, both strands are sequenced.


Send us the nucleic (Genbank accession number) or proteic sequence of your gene of interest. The synthesis of the CpG-free allele will start after you confirm its sequence.
The turnaround time is 2-3 weeks for sequences shorter than 1 kb.
We can process sequences up to 5 kb.


Custom-made CpG-free genes are provided as 20 µg of lyophilized DNA. Products are shipped at room temperature. Store at -20°C.


Product Quantity Catalog Code Price & Order
Custom-made CpG-free Gene 20 µg p-custom contact us
Disclaimer: Our products are provided for research purposes only. Not for use in humans. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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