Bacterial DNA is rich in unmethylated 2’-deoxyribo (cytidine-phosphateguanosine) (CpG) dinucleotides, in contrast to mammalian DNA which contains a low frequency of CpG dinucleotides that are mostly methylated. Unmethylated CpGs in specific sequence contexts activate the vertebrate immune system. Genes with these sequences are recognized as foreign DNA by the vertebrate host leading to a progressive decline of their expression.
InvivoGen develops a family of plasmids completely devoid of CpG dinucleotides, named pCpG. InvivoGen provides 5 families of pCpG plasmids. Many non-mammalian genes are widely used as resistance, reporter or cytotoxic genes in molecular and cellular studies. InvivoGen has synthesized new alleles of these genes, with reduced or no CpG dinucleotides. These synthetic genes display higher activity and lower immunogenicity than their wild-type counterparts.
InvivoGen is an expert in developing CpG-Free genes and now offers a CpG-Free gene custom service. Send us the sequence of your gene of interest, and our specialists will design, synthesize and clone into an expression vector a CpG-Free allele of this gene. We guarantee that the sequence of the synthetic gene will 100% match the designed sequence.