c-di-AMP Fluorinated

c-di-AMP Fluorinated (c-di[2’FdAMP]) is a synthetic analog of cyclic diadenylate monophosphate (c-di-AMP) with a fluorine atom at 2’ position of the nucleosides. c-di-AMP, a bacterial second messenger, is a potent immunostimulant in mammals. It induces production of type I interferons (IFNs) by directly binding to the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes) [1, 2]. The incorporation of fluorine into biologically active molecules is commonly used in medicinal chemistry to improve their metabolic stability or to modulate physicochemical properties such as lipophilicity [3, 4]. Moreover, the introduction of a fluorine atom can change the biological activities of a molecule. Interestingly, when used at low concentrations in various cellular assays, c-di-AMP Fluorinated induces higher levels of type I IFNs than does c-di-AMP.

STING ligands such as c-di-AMP induce type I IFNs and activate interferon stimulated genes (ISG) through interferon regulatory factors (IRFs). To facilitate their study, InvivoGen has developed stable reporter cells in two well established immune cell models: THP-1 human monocytes and RAW 264.7 murine macrophages. These cells express a reporter gene (SEAP or Lucia luciferase), under control of an IRF-inducible promoter.

Figures for this product

IRF INDUCTION (Lucia luciferase reporter)NF-kB INDUCTION (SEAP reporter)


Description: STING agonist
Synonym: 2’Fluoro-c-di-AMP sodium salt, 2',2'-di-F-c-didAMP, c-di(2’FdAMP)
CAS number: 1427269-47-3
Formula: C20H20F2N10O10P2 .2Na
Molecular weight: 706.36
Purity: ≥ 95% by LC/MS & NMR
Solubility: 50 mg/ml in water
Quality control:
- The ability of c-di-AMP Fluorinated to induce type I interferon (IFN) has been confirmed in THP1-Blue™ ISG cells.
- The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.


- 100µg lyophilized c-di-AMP Fluorinated
- 1.5 ml endotoxin-free water

Product is shipped at room temperature and should be stored at -20°C. Product is sterile filtered prior to lyophilization.


c-di-AMP fluorinated


  1. Jin L. et al., 2011. MPYS is required for IFN response factor 3 activation and type I IFN production in the response of cultured phagocytes to bacterial second messengers cyclic-di- AMP and cyclic-di-GMP. J Immunol. 187(5):2595-601.
  2. Burdette DL. et al., 2011. STING is a direct innate immune sensor of cyclic di-GMP. Nature. 478(7370):515-8.
  3. Liu P. et al., 2008. Fluorinated Nucleosides: Synthesis and biological implication. J Fluor Chem. 129(9): 743–766.
  4. Böhm HJ. et al., 2004. Fluorine in medicinal chemistry. Chembiochem. 5(5):637-43.
  5. Unterholzner L. et al., 2010. IFI16 is an innate immune sensor for intracellular DNA.Nat Immunol. 11(11):997-1004.
  6. Zhang Z. et al., 2011. The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat Immunol.12(10):959-65.
  7. Arakawa R. et al., 2010. Characterization of LRRFIP1. Biochem Cell Biol. 88(6):899-906.
  8. Lippmann J. et al., 2010. IFN beta responses induced by intracellular bacteria or cytosolic DNA in different human cells do not require ZBP1 (DLM-1/DAI). Cell Microbiol. 10(12):2579-88.


c-di-AMP Fluorinated

Description STING ligand
Cat. Codetlrl-nacdaf
Unit Size100 µg
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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