Anti-hIL-1α Neutralizing mAb

Neutralizing monoclonal antibody against human interleukin 1 alpha

Neutralizing monoclonal antibody against human IL-1α

Anti-hIL-1α-IgG (clone 7D4) is a monoclonal antibody (mAb) specific for human interleukin 1 alpha (hIL-1α). It is produced in hybridomas and purified by affinity chromatography. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-1α.
IL-1α is a pro-inflammatory cytokine that plays a central role in the regulation of immune responses and inflammation [1]. 

More details

Key features:

• A potent and specific neutralizing mAb against hIL-1α
• Provided azide-free
• No cross-reactivity with human IL-1β, murine IL-1α, or murine IL-1β
• Each lot is functionally tested

Reference:

1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27. 

Figures

Anti-hIL-1α-IgG is a potent neutralization antibody. Increasing concentrations of Anti-hIL-1α-IgG were incubated for 30 minutes with the recombinant cytokine hIL-1α (1 ng/ml) prior to the addition of HEK-Blue™ IL-1β cells. After overnight incubation, SEAP activity in the cell culture supernatant was assessed using QUANTI-Blue™ Solution. Data are shown in percentage (%) of neutralization.

Specifications

Target: Natural and recombinant human interleukin-1 alpha (IL-1α)

Specificity: No cross-reactivity with human IL-1β, murine IL-1α, or murine IL-1β

Clone: 7D4

Isotype: Mouse IgG1, kappa

Control: Mouse IgG1 Control

Immunogen: Human IL-1α protein expressed in Swiss mice following DNA immunization

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Tested applications: Blocking & Neutralisation

Quality control:

• This product has been validated for neutralization.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 3x 100 µg purified anti-hIL-1α-IgG provided lyophilized

 The product is shipped at room temperature.

 Upon receipt, store lyophilized antibody at -20 °C.

Reconstituted antibody is stable for 1 month at 4 °C and for 1 year at -20 °C.

 Avoid repeated freeze-thaw cycles.

Details

Interleukin-1 alpha (IL-1α) is a secreted pro-inflammatory cytokine produced primarily by activated macrophages [1]. IL-1α is synthesized as a pro‑cytokine, which is enzymatically cleaved into the mature secreted form by calpain, a calcium-dependent protease [2]. In contrast to other cytokines, both the precursor and mature forms of IL-1α are biologically active.

IL-1α plays a central role in the regulation of immune responses and inflammation. IL-1α binds to the interleukin-1 receptor, leading to the activation of NF-κB signaling and the JNK and p38 mitogen-activated protein kinase pathways, which induce the expression of inflammatory cytokines and chemokines, such as IL-6 and IL-8 [3].

1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8-27.
2. Sims J. & Smith D., 2010. The IL-1 family: regulators of immunity. Nat Rev Immunol. 10(2):89-102.
3. Weber A. et al., 2010. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.