Anti-β-Gal-hIgG1

Monoclonal human IgG1 antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG1 features the constant region of the human IgG1 isotype and the variable region of Mouse IgG2a Control. Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal).

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice. It can be used as an isotype control for human IgG1 antibodies.

Human IgG1 is the most abundant immunoglobin present in serum and binds with high affinity to the Fc receptor on phagocytic cells.

The human IgG1 isotype displays high antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC).

Anti-β-Gal-hIgG1 was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity  chromatography.

Figures

Comparison of ADCC potency for native and engineered anti-human TIGIT antibody isotypes: Raji-hTIGIT cells were incubated with gradient concentrations of Anti-hTIGIT or Anti-β-galactosidase (β-gal) mAbs for 1 hour. Jurkat-Lucia™ NFAT-CD16 effector cells were then co-incubated with target cells for 6 hours. NFAT activation, reflecting the induced ADCC response, was assessed by determining Lucia luciferase activity in the supernatant using QUANTI-Luc™. Percentages of the maximal response normalized to the IgG1 isotype are shown.

Specifications

Clonality: Monoclonal antibody

Specificity: Targets cells expressing E. coli β-galactosidase (β-Gal)

Isotype: Human IgG1, kappa

Source: CHO cells

Application: Negative control for human IgG1 antibodies

Quality control:

• The absence of binding of Anti-β-Gal-hIgG1 to human cell lines has been controlled using flow cytometry.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

Contents

Anti-β-Gal-IgG1 purified monoclonal antibody is provided azide-free and lyophilized. It is available in two quantities:

• bgal-mab1: 200 µg
• bgal-mab-1: 1 mg

​ Product is shipped at room temperature.

Store lyophilized antibody at -20°C.

Product is stable for 1 year.