DNA methyltransferase inhibitor
5-Aza-2’-deoxycytidine (5-AzadCyD, 5-Aza-CdR, decitabine) is a specific inhibitor of DNA methylation. It functions in a similar manner to aza-citidine, although decitabine can only be incorporated into DNA strands while azacitidine can be incorporated into both DNA and RNA chains.
5-AzadCyD is a prodrug that requires activation via phosphorylation by deoxycytidine kinase. The nucleotide analog is incorporated into DNA, where it produces an irreversible inactivation of DNA methyltransferase. 5-AzadCyD was demonstrated to be a potent antineoplastic agent against leukemia and tumors in animal models [1,2].
Working concentration: 0.1-10 μM
CAS number: 2353-33-5
Molecular weight: 228.2
Solubility: Soluble in acetic acid:water (1:1 v/v) at 50 mg/ml
• 10 mg
1. Christman JK., 2002. 5-Azacytidine and 5-aza-20-deoxycytidine as inhibitors of DNA methylation: Mechanistic studies and their implications for cancer therapy. Oncogene 21, 5483–5495.
2. Kulis M. & Esteller M., 2010. DNA methylation and cancer. Adv Genet. 2010;70:27-56