3’3’-cGAMP VacciGrade™

Cyclic [G(3’,5’)pA(3’,5’)p] - STING ligand

3’3’-cGAMP (cyclic [G(3’,5’)pA(3’,5’)p]) is a cyclic dinucleotide (CDN) produced by bacteria in which it serves as a second messenger and plays a role in chemotaxis and colonization [1]. 3’3’-cGAMP is also referred to as “canonical” cGAMP due the presence of the classical 3’-5’ phosphodiester linkages between the guanosine and the adenosine.

CDNs are a relatively new class of adjuvants that have been shown to increase vaccine potency [2]. CDNs activate innate immunity by directly binding to the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes), activating a signaling pathway that induces the expression of interferon-β (IFN-β) and also nuclear factor-κB (NF-κB) dependent inflammatory cytokines [3]

3’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 3’3’-cGAMP.

3’3’-cGAMP is VacciGrade™ (preclinical grade) for research use only, not for use in humans.

Figures for this product

IRF INDUCTION (Lucia luciferase reporter)NF-kB INDUCTION (SEAP reporter)


Description: STING agonist VacciGrade™
Synonym: Cyclic [G(3’,5’)pA(3’,5’)p], cyclic GMP-AMP; c-GpAp sodium salt
CAS number: 849214-04-6
Formula: C20H22N10O13P2 .2Na
Molecular weight: 718.38
Purity: ≥ 95% by LC/MS
Solubility: 50 mg/ml in physiological water
Quality: Sterile
Endotoxin Level: Lower than 0.005 EU/μg
Biological activity: Assessed by measuring induction of the interferon pathway in THP1-Blue™ ISG cells


• 1 mg (2 x 500 µg) lyophilized 3’3’-cGAMP VacciGrade™
• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

3’3’-cGAMP VacciGrade™ is shipped at room temperature and should be stored at -20°C.


3’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 3’3’-cGAMP. It is prepared under strict aseptic conditions and is tested for the presence of endotoxins. 3’3’-cGAMP VacciGrade™ is guaranteed sterile. Its endotoxin level is lower than 0.005 EU/μg.



1. Davies B. et al., 2012. Coordinated regulation of accessory genetic elements produces cyclic di-nucleotides for V. cholerae virulence. Cell. 149(2):358-70.
2. Dubensky TW. et al., 2013. Rationale, progress and development of vaccines utilizing STING-activating cyclic dinucleotide adjuvants. Therapeutic Advances in Vaccines 1(4): 131-143.
3. Zhang X. et al., 2013. Cyclic GMP-AMP containing mixed phosphdiester linkages is an endogenous high-affinity ligand for STING. Mol Cell.51(2):226-35.


Recent articles using InvivoGen 3’3’-cGAMP VacciGrade™


3’3’-cGAMP VacciGrade™

Description STING ligand - Cyclic [G(3’,5’)pA(3’,5’)p]
Cat. Codevac-nacga
Unit Size1 mg
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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