293T-Dual™ hSTING-A162 CellsNEW

293T-Dual™ hSTING-A162 Cells (ISG-SEAP/KI-[IFN-β]Lucia)

293T-Dual™ hSTING-A162 (ISG/KI-IFNb) cells were generated from 293T-Dual™ Null (ISG/KI-IFNb) cells by stable transfection of the A162 isoform of human STING (S162A). The allele A162 contains a unique point mutation (S162A) placed at the cyclic-dinucleotide-binding site which confers sensitivity to DMXAA, a potent tumor vascular disrupting agent in mice [2]. In the absence of this mutation, DMXAA has no effect on human STING [3].

293T-DualhSTING-A162 (ISG/KI-IFNb) cells are resistant to blasticidinhygromycin and Zeocin™ .

Figures for this product

IRF induction (SEAP reporter)IFN-β induction (Lucia luciferase reporter)


Antibiotic resistance: blasticidinhygromycin, and Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Quality Control:

  • Reporter activity has been validated by stimulating the cells with human interferon-β (hIFN-β) and IRF3 activators, such as c-di-AMP and cGAMP.
  • The biallelic replacement of the hIFN-β coding sequence with the Lucia luciferase open reading frame (ORF) has been verified by PCR and sequencing. Furthermore, the inability to produce IFN-β has been confirmed by ELISA.
  • The cell line stability for 20 passages following thawing has been verified.

Guaranteed mycoplasma-free

Shipped on dry ice


  • 1 vial of 293T-Dual™ hSTING-A162 (ISG/KI-IFNb) Cells (3-7 x 106 cells)
  • 100 μl Blasticidin (10 mg/ml). Store at 4 °C or at -20 °C.*
  • 100 μl Hygromycin (100 mg/ml). Store at 4 °C or at -20 °C.*
  • 100 μl Zeocin™ (100 mg/ml). Store at 4 °C or at -20 °C.*
  • 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of 3 antibiotics active against mycoplasmas, bacteria and fungi. Store at -20 °C.*
  • 1 pouch of QUANTI-Blue™ (SEAP detection medium).
    Store QUANTI-Bluepouch at 4 °C for 6 months. Reconstituted QUANTI-Blue™ medium is stable for 2 weeks at 4 °C. Protect from light.
  • 1 pouch of QUANTI-Luc™.
    Store QUANTI-Lucpouch at -20 °C for 12 months. Reconstituted QUANTI-Luc™ medium is stable for 1 week

* The expiry date is specified on the product label.


  1. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLoS One. 8(10):e77846.
  2. Gao P. et al., 2013. Structure-function analysis of STING activation by c[G(2’,5’)pA(3’,5’)p] and targeting by antiviral DMXAA. Cell 154(4):748-62.
  3. Conlon J. et al., 2013. Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid. J Immunol 190(10):5216-25.


293T-Dual™ hSTING-A162 CellsNEW!

Description Dual IRF and IFN-β reporter 293T cells expressing A162 isoform of human STING (S162A)
Cat. Code293d-a162
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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