293XL/hTLR7-HA cells were obtained by stable transfection of HEK293XL cells with the pUNO-hTLR7-HA plasmid which expresses the human TLR7 gene fused at the 3'end to the influenza hemaglutinine (HA).

The control cell line for 293XL/hTLR7-HA cells is 293XL/null cells.


Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl Blasticidin (10 mg/ml)

1 ml Normocin™ (50 mg/ml)


TLR7, which is abundantly expressed in lung, placenta, spleen and PBL, is phylogenetically close to TLR8 and TLR9 [1]. TLR7 recognizes small synthetic molecules such as loxoribine and R848, an imidazoquinoline compound [2]. TLR7 signaling involves the MyD88-dependent signaling cascade and induces the production of IFN-α, TNF-α and IL-12. Recently, single-stranded RNA (ssRNA) was identified as the natural ligand of TLR7 [3, 4]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in PDC. TLR7 signaling is abrogated by chloroquine indicating that it is dependent on endosomal acidification.

1. Chuang TH. and RJ. Ulevitch, 2000. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw, 11(3):372-8
2. Hemmi H. et al., 2002. Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway. Nat Immunol, 3(2):196-200
3. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 303(5663):1526-9.
4. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 303(5663):1529-31.


Recent articles using InvivoGen 293XL/hTLR7-HA



Description 293XL cell line expressing the HA-tagged human TLR7 gene
Cat. Code293xl-htlr7ha
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".